Composite

Part:BBa_K2688024:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)


tetO_RBS_ler3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene ler3 encodes the Ler protein of E. coli 3030-1 (GenBank: EGW78798.1) with a point mutation (A 101 ->T ; encoding Ler3 K34M)


Source

The gene ler3 is a spontaneous mutant (A 101 ->T ; encoding Ler2 K34M) obtained during the cloning of ler2 gene (BBa_K2688019)


The gene ler1 was sequence-optimized and synthetized. Original sequence from (Leh et al. mBIO, 2017).

References